Hematology testing machine DH36

DH36 hematology testing machine is a device used in clinical practice to analyze blood cells, differentiate 3 white blood cell components, and measure hemoglobin concentration.

Operating principle: The hematology analyzer uses the electrical impedance method to analyze WBC, RBC and PLT and their distribution volumes; Colorimetric method is used to measure hemoglobin concentration.

• Principle of electrical impedance method: WBCs/RBCs/PLTs are counted and sized based on the electrical impedance method. The method works by measuring the change in resistance created by a blood cell as it passes through an aperture of known size. The electrode is placed at both ends of the solution on both sides of the aperture to form an electrically conductive path. As each cell passes through the aperture it creates a change in the resistance between the two electrodes. This change produces an electrical pulse that can be measured. The number of electrical pulses generated is equal to the number of cells passing through the aperture, and the amplitude of each electrical pulse is proportional to the volume of the corresponding cell. Each electrical pulse is amplified and compared to the internal reference voltage channel, and only electrical pulses with sufficiently high amplitude are accepted. If the electrical pulse generated has an amplitude higher than the lower threshold value of WBC/RBC/PLT, it is considered WBC/RBC/PLT.
• Principle of colorimetric method: WBC/HGB diluent is introduced into the HGB bath and mixed with a certain amount of lysate, converting hemoglobin into a complex that can be measured at 525nm. An LED is mounted on one side of the tank and emits a monochromatic beam of light at a wavelength of 525 nm. Light passes through the sample and is measured by an optical probe on the opposite side. The signal is then amplified and the voltage is measured and compared to a blank sample (sample measured when there is only dilute solution in the tank).

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