DH615 automatic hematology analyzer DH615

The automatic hematology analyzer is a device used clinically to analyze blood cells, differentiate the 5 components of white blood cells, measure hemoglobin concentration and measure reticulocytes.

Analysis modes: DH-615 has 5 analysis modes: CBC (complete blood count), RET (reticulocyte count), CBC+DIFF (white blood cell differentiation), CBC+RET and CBC+DIFF +RET.

Operating principle: The automated hematology analyzer uses the electrical impedance method (also known as the Coulter Principle), the fluorescent dye method and the semiconductor laser-scattering flow cytometry method. based flow cytometry) to count and differentiate blood cells; Colorimetric method is used to measure hemoglobin concentration.

Principle of electrical impedance method: The analysis sample is put into the white blood cell analysis unit (or red blood cell/platelet analysis unit) after being diluted. The method works by measuring the change in resistance created by a blood cell as it passes through an aperture of known size. The electrode is placed at both ends of the solution on both sides of the aperture to form an electrically conductive path. As each cell passes through the aperture it creates a change in the resistance between the two electrodes. This change produces an electrical pulse that can be measured. The number of electrical pulses generated is equal to the number of cells passing through the aperture, and the amplitude of each electrical pulse is proportional to the volume of the corresponding cell. Each electrical pulse is amplified and compared to the internal reference voltage channel, and only electrical pulses with sufficiently high amplitude are accepted. If the electrical pulse generated has an amplitude higher than the lower threshold value  of WBC/RBC/PLT, it is considered WBC/RBC/PLT.

Principle of fluorescent staining method: During the dilution process, the testing machine will dye and mark nucleic acids in cells. The purity of nucleic acids varies between different cell types, at different stages or at unusual stages of development, leading to different concentrations of fluorescent dyes. When light shines on fluorescent materials such as fluorescently dyed blood cells, it produces light with a longer wavelength. The higher the dye concentration, the higher the fluorescence level. By measuring the level of fluorescence, information about the degree of cell staining is obtained.

Principle of semiconductor laser scattering flow cytometry method: A certain amount of blood cells is injected into a conical flow chamber containing a dilute solution after being fluorescently stained. The colored cells are fed through the center of the flow chamber in a column at high speed, and are passed through a laser that causes the light to scatter at many different angles. The optical probe will receive prescatter, side fluorescence and side scatter signals and convert them into electrical pulse signals. Electrical pulses are used to plot 3D scatterplots of blood cell size, cell internal information, and fluorescence levels. In which, forward scatter (FSC) shows the difference in blood cell size, side scatter (SSC) shows the complexity of particles inside blood cells, and side fluorescence shows the level of staining of blood cells. cell.

Principle of colorimetric method: WBC/HGB diluent is introduced into the HGB tank and mixed with a certain amount of lysate, converting hemoglobin into a complex that can be measured at 525 nm. An LED is mounted on one side of the tank and emits a beam of monochromatic light at a wavelength of 525 nm. The light passes through the sample and is measured by the optical probe on the opposite side. The signal is then amplified and the voltage is measured and compared to a blank sample (sample measured when there is only dilute solution in the tank).

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